Substrate deformation determines actin cytoskeleton reorganization: A mathematical modeling and experimental study

A mathematical model has been developed to define the relationship between the actin cytoskeleton reorganization of a cell and substrate deformation a cting on the cell. The model is based on the following major assumptions: ( a) normal substrate strain, not the shear substrate strain, determines the actin cytoskeleton reorganization; (b) the normal substrate strain is trans mitted to individual actin filaments; (c) each actin filament has a basal s train energy (BSE) when the cell adheres to the substrate without stretchin g; and (d) the actin filaments undergo disassembly when their strain energi es are decreased to zero or increased to twice their BSEs. The resulting mo del predicts that the actin filaments are formed in the direction where the ir BSEs are minimally altered. This direction is therefore the one without normal substrate strain. The prediction was confirmed by experiments conduc ted on both fibroblasts and endothelial cells. The present model may be rel evant for understanding better the effects of mechanical stimuli on the cel ls. (C) 2000 Academic Press.