Mechanism of assembly of recombinant murine polyomavirus-like particles

Authors
Schmidt, U Rudolph, R Bohm, G
Citation
U. Schmidt et al., Mechanism of assembly of recombinant murine polyomavirus-like particles, J VIROLOGY, 74(4), 2000, pp. 1658-1662
Citations number
19
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
74
Issue
4
Year of publication
2000
Pages
1658 - 1662
Database
ISI
SICI code
0022-538X(200002)74:4<1658:MOAORM>2.0.ZU;2-1
Abstract
VP1 is the major viral coat protein of murine polyomavirus and can be used for the generation of virus-like particles in vitro. Here, me demonstrate t hat capsid assembly is an equilibrium reaction followed by oxidation of int racapsomere disulfide bonds, which are not essential for the formation of v irus-like particles but enable complete particle assembly and prevent capsi d dissembly.