M. Fredriksson-ahomaa et al., Efficient subtyping of Yersinia enterocolitica bioserotype 4/O : 3 with pulsed-field gel electrophoresis, LETT APPL M, 29(5), 1999, pp. 308-312
The purpose of this study was to evaluate the efficiency of pulsed-field ge
l electrophoresis (PFGE) for differentiation of isolates of Yersinia entero
colitica bioserotype 4/O:3 recovered from pig tongues at retail level in th
e Helsinki area during October and November of 1996. NotI generated 15 diff
erent PFGE patterns for 128 isolates. The discriminatory index did not exce
ed 74% due to the presence of two predominant PFGE-types, NA1 (58/128) and
NB1 (25/128). After preliminary investigations with 35 enzymes, ApaI, XbaI,
XhoI and SpeI were chosen for further characterization of the isolates. Th
e discriminatory index increased from 74% to 93% and the number of differen
t genotypes from 15 to 30 when isolates with the same PFGE pattern with Not
I were further characterized with ApaI and XhoI, indicating that PFGE can b
e an efficient technique for characterization of bioserotype 4/O:3.