The profile of the principal free fatty acids (FFA) in Manchego cheese thro
ughout ripening was determined. The FFAs that increased most were C-4:0, C-
6:0 and C-8:0. The FFA that best correlated with ripening time were the sho
rt-chain fatty acids. Short-chain fatty acids percentages were lower in the
triglyceride fraction than in the FFA fraction at the beginning of maturat
ion. As ripening time progressed these acids tended to reach similar levels
in both fractions.