Oligonucleotide-conjugated beads for transdominant genetic experiments

Transdominant genetics using expression libraries can identify proteins and peptides that affect cell division, In conjunction with these libraries, o ligonucleotide-conjugated beads and flow cytometry were used to test a stra tegy that potentially expands the range of such genetic studies, The experi mental approach involved creation of tagged expression libraries, introduct ion of these libraries into cells, growth of the cultured cells for several generations and recovery on oligonucleotide-conjugated beads of sequences that encode growth-modulatory proteins or peptides, Experiments in Saccharo myces cerevisiae demonstrating the feasibility of the strategy are presente d.