Histochemical localization of glutathione in plant tissues: A comparison of two fluorescence staining methods

Authors
Muller, M Tausz, M Guttenberger, H Grill, D
Citation
M. Muller et al., Histochemical localization of glutathione in plant tissues: A comparison of two fluorescence staining methods, PHYTON AUST, 39(3), 1999, pp. 69
Citations number
21
Categorie Soggetti
Plant Sciences
Journal title
PHYTON-ANNALES REI BOTANICAE
ISSN journal
00792047 → ACNP
Volume
39
Issue
3
Year of publication
1999
Database
ISI
SICI code
0079-2047(1999)39:3<69:HLOGIP>2.0.ZU;2-H
Abstract
Two fluorescent dyes monochlorobimane and mercury orange that label glutath ione (GSH) were used as a tool for measuring the intracellular distribution of GSH in living epidermal cells of Allium cepa. Both agents gave repeatab le staining which could be subjected to quantitative image analysis. Blocka ge of thiol groups with N-ethylmaleimide (NEM) decreased fluorescence of bo th dyes to a similar extent demonstrating specifity to SH groups.