Use of green fluorescent protein for detection of transformed shoots and homozygous offspring

Authors
Molinier, J Himber, C Hahne, G
Citation
J. Molinier et al., Use of green fluorescent protein for detection of transformed shoots and homozygous offspring, PL CELL REP, 19(3), 2000, pp. 219-223
Citations number
18
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
07217714 → ACNP
Volume
19
Issue
3
Year of publication
2000
Pages
219 - 223
Database
ISI
SICI code
0721-7714(200001)19:3<219:UOGFPF>2.0.ZU;2-X
Abstract
Using tobacco as a model system, the data obtained demonstrated that the gr een fluorescent protein (GFP) can be used as a visual selection marker for transformed tissues. Based on differences in the intensity of GFP fluoresce nce, homozygous and hemizygous states could be easily visualized in seeds a nd seedlings of the T1 generation. These results were confirmed by genetic analysis. Optimized conditions for GFP analysis of stable transformants are presented.