Effect of thidiazuron on bud development of Cymbidium sinense Willd in vitro

Seed-derived rhizomes of Cymbidium sinense Willd were able to initiate shoo t buds in the presence of 0.01-1 mg/l thidiazuron (TDZ). However, TDZ retar ded rhizome proliferation. The regenerated shoot buds which had been cultur ed on basal medium with a supplement of 0.5 mg/l 1-naphthalene acetic acid (NAA) and 1 g/l active charcoal were able to form 10 cm plantlets within fo ur months. Plantlets were transplanted to sphagnum moss and cultured on a m ist bench for six months and then planted into soil-filled pots and maintai ned in the greenhouse, where they grew well. In the 2nd year, 9.1% of the p otted plants bore normal flowers. In the 3rd year, each of them produced 5- 6 shoots about 43 cm in height and about 20 roots; 73% of the potted plants flowered normally.