Detection of tobacco rattle virus in potato tubers using a simple RT-PCR procedure

Authors
Nicolaisen, M Bosze, Z Nielsen, SL
Citation
M. Nicolaisen et al., Detection of tobacco rattle virus in potato tubers using a simple RT-PCR procedure, POTATO RES, 42(2), 1999, pp. 173-179
Citations number
23
Categorie Soggetti
Agriculture/Agronomy
Journal title
POTATO RESEARCH
ISSN journal
00143065 → ACNP
Volume
42
Issue
2
Year of publication
1999
Pages
173 - 179
Database
ISI
SICI code
0014-3065(1999)42:2<173:DOTRVI>2.0.ZU;2-5
Abstract
A simple assay for detection of tobacco rattle virus in infected tubers inv olving RT-PCR was developed. The assay detected a wide range of strains, in cluding nonparticle NM variants. RNA extraction methods were compared. and a simple method was found to give reliable results. The method enabled dete ction of at least 1 ng of tobacco rattle particles in 100 mg tuber extract.