Md. Noseworthy et Tm. Bray, Zinc deficiency exacerbates loss in blood-brain barrier integrity induced by hyperoxia measured by dynamic MRI, P SOC EXP M, 223(2), 2000, pp. 175-182
Citations number
41
Categorie Soggetti
Medical Research General Topics
Journal title
PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE
Using dynamic Magnetic Resonance Imaging (dMRI), blood-brain barrier (BBB)
permeability (k(PS rho)) and tissue interstitial leakage space (v(e)) were
evaluated in zinc-deficient (ZnDF) male weanling Wistar rats following 3 da
ys exposure to hyperoxia (85% O-2). Temporal monitoring of T1-weighted MR i
mage changes, following a bolus intravenous injection of gadolinium-DTPA, a
llowed estimation of BBB integrity, Three-day exposure of hyperoxia caused
a marginal loss of BBB integrity, reflected in a slight increase in k(PS rh
o) and v(e), observed in both the animals fed adequate zinc (ZnAL) and pair
-fed controls (ZnPF), However, zinc deficiency resulted in a significant in
crease in both k(PS rho) and v(e), indicating a severely disturbed BBB, In
addition MR-visible free water was elevated in ZnDF brains following hypero
xia treatment indicating that a loss of BBB integrity may be associated wit
h neuronal edema. The diminished BBB integrity may be free-radical mediated
as the ratio of oxidized to reduced glutathione (GSSG:GSH) was significant
ly elevated.