Aa. Tomilov et al., Insertional mutagenesis in Arabidopsis thaliana: Presonication of germinating seeds increases the efficiency of their transformation, RUSS J GEN, 35(9), 1999, pp. 1043-1049
An efficient procedure of transforming Arabidopsis thaliana germinating see
ds was elaborated on the basis of the method of Feldmann and Marks. The pro
cedure involves microdamaging TI seeds by sonication before culturing with
a vector Agrobacterium strain and yields more than 1% T2 transformants. Ger
minating seeds were transformed with Agrobacterium tumefaciens hypervirulen
t strain A281 carrying plasmid pLD3 derived from pBI121. A collection of 54
A. thaliana T2 transformants was obtained; some of them showed marked morp
hological alterations. The transgenic nature of plants that acquired resist
ance to a marker antibiotic was confirmed histochemically and by PCR amplif
ication of a T-DNA region.