Av. Kukekova et al., Cloning and expression of the gene for mycoplasma key cell-division protein ftsZ in Escherichia coli, RUSS J GEN, 35(3), 1999, pp. 249-255
Gene ftsZ responsible for division of bacterial cells was revealed in most
prokaryote groups. A 520-bp fragment of the ftsZ gene was amplified on the
template of A. laidlawii DNA using degenerate primers. This fragment was se
quenced and served as a hybridization probe for cloning of the full-sized c
opy of the A. laidlawii ftsZ gene. The amplified fragment was cloned in a p
GEX3X vector and expressed in E. coli cells. Polyclonal antibodies derived
from the chimeric polypeptide containing a fragment of A. laidlawii FtsZ pr
otein interacted only with the A. laidlawii protein with molecular mass of
40 kDa. Comparison of nucleotide sequences of the ftsZ-gene region of A. la
idlawii and other bacterial species showed that they were highly homologous
in A. laidlawii, E. coli, and Bac. subtilis, while low homology was reveal
ed between the A. laidlawii sequence and those of the members of the genus
Mycoplasma. Analysis of the ftsZ-gene nucleotide sequences is suggested as
a means to study the evolutionary relatedness of prokaryotes.