Four new human renal cell carcinoma cell lines expressing globe-series gangliosides

Clinicopathological studies revealed that monosialosyl galactosyl globoside (MSGG) and disialosyl galactosyl globoside (DSGG) expressed by renal cell carcinoma (RCC) are one of the biochemical indicator related to the metasta tic potential. The present study examines the characteristics of four new h uman RCC cell lines and compares the expression of MSGG and DSGG among them using TLC immunostaining and flow cytometry. TOX-1 and TOX-2 were derived from metastatic subcutaneous tissues. TOS-3 and TOX-3LN mere derived from t he primary lesion and from metastatic lymph nodes respectively. Monolayer c ulture, light microscopy and electron microscopy of these cells showed that these cell lines were derived from RCC. TLC immunostaining and flow cytome tric analysis revealed increased levels of MSGG in TOX-2 and TOS-3LN, and i ncreased DSGG in TOS-1 and TOS-3LN. These cell lines mould be useful for fu nctional studies of globe-series ganglioside expressed by RCC. (C) 1999 Toh oku University Medical Press.