The integration of SPR biosensors with mass spectrometry: possible applications for proteome analysis

The successful integration of biospecific interaction analysis based on sur face plasmon resonance and mass spectrometry produces a powerful technique that couples the benefits of sensitive affinity capture and characterizatio n of binding events with the ability to characterize interacting molecules. A variety of biosensors has been used to capture proteins and peptides bio specifically on sensor surfaces, with subsequent analysis using mass spectr ometry. Applying this type of analysis to proteomic studies could lead to l igand and protein-complex identification, and might provide clues leading t o the identification of pathways.