Crystallization and preliminary crystallographic studies of ribosome recycling factor from Escherichia coli

Authors
Yun, J Kim, W Ha, SC Eom, SH Suh, SW Kim, KK
Citation
J. Yun et al., Crystallization and preliminary crystallographic studies of ribosome recycling factor from Escherichia coli, ACT CRYST D, 56, 2000, pp. 84-85
Citations number
12
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
56
Year of publication
2000
Part
1
Pages
84 - 85
Database
ISI
SICI code
0907-4449(200001)56:<84:CAPCSO>2.0.ZU;2-H
Abstract
Ribosome recycling factor (RRF) catalyzes the disassembly of a termination complex during the final stage of protein synthesis. RRF from Escherichia c oli has been crystallized with PEG 400 as precipitant at 287 K. The crystal belongs to the trigonal space group P3(1)21 (or P3(2)21), with unit-cell p arameters a = b = 48.08, c = 141.67 Angstrom. Native data were collected fr om a frozen crystal to a resolution of 3.0 Angstrom on a Cu K alpha rotatin g-anode X-ray source.