Crystallization and preliminary X-ray diffraction analysis of the NAD-dependent non-phosphorylating GAPDH of the hyperthermophilic archaeon Thermoproteus tenax

Recombinant non-phosphorylating NAD(+)-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN) of the hyperthermophilic crenarchaeote Thermoproteus tenax has been overexpressed, purified and crystallized using the hanging-d rop vapour-diffusion technique. Crystals of different habits were obtained from several precipitant solutions (salts and polyethylene glycols). Prelim inary X-ray analysis was performed with crystals grown in ammonium formate, which belonged to the primitive hexagonal space group P622, and had unit-c ell parameters a = b = 184.8, c = 133.0 Angstrom, gamma = 120 degrees. Assu ming a molecular weight of 55 kDa, a Matthews parameter of 3.3 Angstrom(3) Da(-1) is calculated assuming two molecules per asymmetric unit. The diffra ction limit of these crystals 2.5 Angstrom resolution.