Serum soluble HLA-DR antigens in autoimmune hepatitis

To investigate the significance of HLA-class II, especially DR antigens, in autoimmune hepatitis (AIH), the serum concentrations of soluble HLA-DR ant igen (sDR) were measured in 16 patients with AIH. The expression of HLA-DR antigens in the liver tissues of AIH patients was also studied by immonohis tochemistry. AIH at diagnosis showed markedly higher serum sDR levels than controls, in which the liver tissues exhibited positive staining of HLA-DR antigens, Seven patients received corticosteroid therapy, in whom the serum sHLA-DR c oncentration was reduced dramatically from activated to remission stage, In sequentially follow-np cases, sDR correlated well with the disease activit y, and also with the change of surface DR expression in the liver. A single major band with a molecular size of 60 kDa was detected, both in patient's sera and in normal control sera, by Western blotting. In conclusions, serum sHLA-DR level could be a marker reflecting immunologi cal activity of the disease.