K. Naruse et al., Anabolic response of mouse bone-marrow-derived stromal cell clone ST2 cells to low-intensity pulsed ultrasound, BIOC BIOP R, 268(1), 2000, pp. 216-220
Citations number
35
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
The effects of 20-min exposure to low-intensity, pulsed ultrasound were inv
estigated in ST2 cells of bone marrow stromal origin. They responded to ult
rasound with elevated levels of IGF mRNAs, osteocalcin, and bone sialoprote
in mRNAs. The upregulated expression of these messages appeared in a biphas
ic manner, with the first peak resistant to the protein synthesis inhibitor
cycloheximide, and a second peak that was eliminated by NS398, an inhibito
r of the inducive prostaglandin G/H synthase (cyclooxygenase-2). A cumulati
ve effect of mechanical loading called the memory effect, which has been ob
served in vivo, can be explained from such a biphasic anabolic reaction med
iated by prostaglandins. The upregulation of IGF or osteocalcin mRNAs can b
e observed even at 24 h after the initiation of the 20-min exposure to ultr
asound. Our results suggest that this low-intensity, pulsed ultrasound, whi
ch has been clinically used to accelerate the healing processes of fracture
d bone, induces a direct anabolic reaction of osteogenic cells, leading to
bone matrix formation. (C) 2000 Academic Press.