Direct interaction between emerin and lamin A

Emerin is the protein of the inner nuclear membrane that is affected by mut ation in X-linked Emery-Dreifuss muscular dystrophy. The autosomal dominant form of the disease is caused by mutations in the lamin A/C gene. Several lines of circumstantial evidence have suggested an interaction of emerin wi th lamins in the nuclear lamina but direct interaction between the two prot eins has not yet been demonstrated, me now demonstrate direct interaction b etween recombinant emerin and lamin A molecules using biomolecular interact ion analysis (BIA) and monoclonal antibodies. An emerin-lamin A interaction system may be related in function to the LAP2-lamin B system at the inner nuclear rim. (C) 2000 Academic Press.