A conditioning lesion promotes in vivo nerve regeneration in the contralateral sciatic nerve of rats

A conditioning lesion in the sciatic nerve increases in vivo axonal regener ation in the nerve after a second transection. We studied whether this incr eased regeneration also occurs in the contralateral nerve. The left sciatic nerve was transected and sutured in Wistar rats; the nerve was exposed but not transected in controls. After 5 days, the right sciatic nerves of all rats were transected and sutured, Neuronal regeneration was measured at 0, 1, 3, 5, and 7 days with the pinch test and histological staining. IL-1 bet a and TGF-beta 1 expression was also measured. The initial delay in the exp erimental group was significantly shorter, but the regeneration rates were the same. The expression of IL-1 beta and TGF-beta 1 in the right dorsal ro ot ganglia was significantly higher in the experimental group. Nerve injury enhances cytokine expression in the contralateral dorsal root ganglion and promotes contralateral nerve regeneration in vivo by shortening the initia l delay. (C) 2000 Academic Press.