Mechanism of action of aspartyl proteases. VII. Noncovalent complexes of HIV-1 aspartic protease with substrate and substrate-like inhibitor

Citation
Me. Popov et al., Mechanism of action of aspartyl proteases. VII. Noncovalent complexes of HIV-1 aspartic protease with substrate and substrate-like inhibitor, BIOORG KHIM, 25(12), 1999, pp. 911-922
Citations number
27
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANICHESKAYA KHIMIYA
ISSN journal
01323423 → ACNP
Volume
25
Issue
12
Year of publication
1999
Pages
911 - 922
Database
ISI
SICI code
0132-3423(199912)25:12<911:MOAOAP>2.0.ZU;2-B
Abstract
A computer model of a noncovalent complex of HIV-1 aspartyl protease with s ubstrate-like inhibitor JG-365 was a priori constructed by using the approa ches of theoretical conformational analysis and molecular mechanics. The ro ot mean square deviation of the calculated conformation of the inhibitor fr om the X-ray diffraction analysis data was 0.87 Angstrom. These results enabled the a priori calculation of the structure of noncoval ent complex of HIV-1 protease with a hexapeptide fragment of its native spe cific substrate Ser-Gln-Asn-Tyr-Pro-Ile-Val. The only possible orientation of the cleavable peptide bond in this and the nucleophilic water molecule r elative to the catalytically active Asp residues of the enzyme (Asp25 and A sp125) was found that provides for the chemical transformation of the subst rate to a tetrahedral intermediate. An action mechanism of enzymes of this class was proposed on the basis of the analysis of calculated distances. We showed that neither steric distortion of the cleavable bond nor the format ion of unfavorable contacts in molecules of the enzymes and their substrate s accompany the optimum orientation of substrate molecules at the active si tes of HIV-1 aspartyl proteases and rhizopuspepsin.