The Naja oxiana venom cytotoxin II spin-labeled at Lys35 for the EPR studyof its interaction with phospholipid membranes

Authors
Utkin, YN Dubovskii, PV Dubinnyi, MA Zharavin, VA Simonova, TN Barsukov, LI Arseniev, AS
Citation
Yn. Utkin et al., The Naja oxiana venom cytotoxin II spin-labeled at Lys35 for the EPR studyof its interaction with phospholipid membranes, BIOORG KHIM, 25(12), 1999, pp. 930-932
Citations number
6
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANICHESKAYA KHIMIYA
ISSN journal
01323423 → ACNP
Volume
25
Issue
12
Year of publication
1999
Pages
930 - 932
Database
ISI
SICI code
0132-3423(199912)25:12<930:TNOVCI>2.0.ZU;2-N
Abstract
The modification of cytotoxin II from Naja oxiana by N-succinimide ester of 2,2,5,5-tetramethylpyrroline-N-oxyl-3-carboxylic acid led to a mixture of spin-labeled derivatives of this toxin. One of these derivatives, purified to a homogenous state by successive ion-exchange chromatography and reverse -phase HPLC, was shown to contain a single label at Lys35 and to be usable in the EPR studying of the cytotoxin II interaction with phospholipid membr anes.