A microfluidic cartridge to prepare spores for PCR analysis

Authors
Belgrader, P Okuzumi, M Pourahmadi, F Borkholder, DA Northrup, MA
Citation
P. Belgrader et al., A microfluidic cartridge to prepare spores for PCR analysis, BIOSENS BIO, 14(10-11), 2000, pp. 849-852
Citations number
15
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOSENSORS & BIOELECTRONICS
ISSN journal
09565663 → ACNP
Volume
14
Issue
10-11
Year of publication
2000
Pages
849 - 852
Database
ISI
SICI code
0956-5663(200001)14:10-11<849:AMCTPS>2.0.ZU;2-E
Abstract
A prototype cartridge system is described that rapidly disrupts Bacillus sp ores by sonication, adds PCR reagent to the disrupted spores. and dispenses the mixture into a PCR tube. The total time to automatically process the s pores in the cartridge and then detect the spore DNA by real-time PCR was 2 0 min. (C) 2000 Elsevier Science S.A. All rights reserved.