Peroxygenase is an enzyme that can convert a double bond to an epoxide. Per
oxygenase activity from oat (Avena sativa) seeds was immobilized on synthet
ic membranes. The immobilized preparation was tested on oleic acid in aqueo
us and heptane media. The order of oxidant activity was tert-butyl hydroper
oxide > cumene hydroperoxide > H2O2-Urea-H2O2. The immobilized preparation
could be reused. Oleic acid was a preferred substrate compared to its trans
analogue, elaidic acid.