Ah. Thompson et al., Heterogeneous cytokine production by acutely stimulated bronchoalveolar T lymphocytes in reovirus 1/lang-infected mice, CELL IMMUN, 198(2), 1999, pp. 120-130
While the in vitro properties of CD4(+) and CD8(+) cytokine-producing lymph
ocytes have been well studied, the in vivo cytokine production patterns and
relative roles of CD4(+) and CD8(+) T lymphocytes during a primary in vivo
immune response remain unclear, In this study, mice were inoculated intran
asally with reovirus 1/L, and respiratory T lymphocyte populations were ana
lyzed using multicolor flow cytometric analysis for the production of cytok
ine within and between classical type 1/type 2 patterns. Cytokine productio
n observed in vivo following infection did not correlate with classical T c
ell cytokine expression patterns; instead, multiple types of lymphocyte pop
ulations that produced one of several possible cytokine combinations were p
resent. Cytokine production by CD4(+) lymphocytes appears in the early and
middle stages of the immune response, while CD8(+) lymphocytes produce more
cytokine in the later stages. Early cytokine responses occurred predominan
tly in the whole lung and lung-associated lymph node populations. The compl
ex patterns of cytokine expression seen in this study likely influence loca
l cell-mediated immunity as well as the complex interaction of T cell subse
ts and the interaction of T cells with B cells which are necessary for the
generation of cell-mediated and humoral immune responses required for effec
tive broad-spectrum immunity. (C) 1999 Academic Press.