Six durum wheat (Triticum turgidum L.) cultivars were pollinated by Cando/V
eery to produce chromosome 1B, T1BL.1RS F-1 heterozygotes. Each F-1 combina
tion was pollinated by its respective durum maternal parent to yield the fi
rst backcross (BC1) derivatives. Heterozygous 1B, T1BL.1RS plants were iden
tified by glucose phosphate isomerase (GPI) electrophoresis and Giemsa C-ba
nding. These BC1F1 heterozygotes were backcrossed further to their durum pa
rents to yield BC2 derivatives, which were similarly advanced to BC7 and th
en self-pollinated. From the selfed progeny, plants homozygous for chromoso
me 1B and T1BL.1RS were identified biochemically and cytologically. We disc
uss here the utility and uniqueness of this germplasm to elucidate the cont
ributions of the 1RS translocated chromosome arm in different durum genotyp
es.