Chromatographic properties of chiral stationary phases based on human serum albumin. Influence of the polymer sublayer

Chiral stationary phases based on human serum albumin were prepared by usin g a reactive polymer, polyacryloyl chloride, to attach the protein to the s ilica particles. The retention data of the enantiomers depend on the struct ure of the polymer sublayer. In most cases, higher selectivity values are o bserved when the polymer coating is formed by polymerization inside the por es of the silica (in situ polymerization) than with polymer layers deposite d by conventional coating methods. This result was attributed to the higher mobility of polymer chains obtained by the former method, which results in lower distortion of the protein after immobilization.