Segmental expression of Hoxb2 in r4 requires two separate sites that integrate cooperative interactions between Prep1, Pbx and Hox proteins

Direct auto- and cross-regulatory interactions between Hox genes serve to e stablish and maintain segmentally restricted patterns in the developing hin dbrain, Rhombomere r4-specific expression of both Hoxb1 and Hoxb2 depends u pon bipartite cis Hox response elements for the group 1 paralogous proteins , Hoxa1 and Hoxb1. The DNA-binding ability and selectivity of these protein s depend upon the formation of specific heterodimeric complexes with member s of the PBC homeodomain protein family (Pbx genes). The r4 enhancers from Hoxb1 and Hoxb2 have the same activity, but differ with respect to the numb er and organisation of bipartite Pbx/Hox (PH) sites required, suggesting th e intervention of other components/sequences. We report here that another f amily of homeodomain proteins (TALE, Three-Amino acids-Loop-Extension: Prep 1, Meis, HTH), capable of dimerizing with Pbx/EXD, is involved in the mecha nisms of restricted expression. We show that: (1) the r4-specific Hoxb1 and Hoxb2 enhancers are complex elements containing separate PH and Prep/Meis (PM) sites; (2) the PM site of the Hoxb2, but not Hoxb1, enhancer is essent ial in vivo for r4 expression and also influences other sites of expression ; (3) both PM and PH sites are required for in vitro binding of Prep1-Pbx a nd formation and binding of a ternary Hoxb1-Pbx1a (or 1b)-Prep1 complex. (4 ) A similar ternary association forms in nuclear extracts from embryonal P1 9 cells, but only upon retinoic acid induction. This requires synthesis of Hoxb1 and also contains Pbx with either Prep1 or Meis1. Together these find ings highlight the fact that PM sites are found in close proximity to bipar tite PH motifs in several Hox responsive elements shown to be important in vivo and that such sites play an essential role in potentiating regulatory activity in combination with the PH motifs.