INCREASE IN PERCUTANEOUS MUSCLE BIOPSY YIELD WITH A SUCTION-ENHANCEMENT TECHNIQUE

The percutaneous muscle biopsy technique is used in clinical practice and biomedical research. We developed a new enhanced-suction technique [suction-enhancing nipples (SEN)I and compared it with techniques cur rently in practice by assessing biopsy yields on anesthetized pigs. We applied the enhanced-suction technique to human subjects participatin g in a clinical trial. In the pig, there was a mean 91% (1.9-fold) inc rease in the size of the samples obtained with the 4-mm needle when SE N was used and a mean 507% (fivefold) increase in sample size when the SEN was applied to the 6-mm needles. Nine passes of the 6-mm needle w ith SEN obtained from five consecutive human subjects yielded a mean i ndividual sample size of 109.4 mg or 219.4 mg per needle pass when usi ng the double-sample technique. Adequate tissue samples for histomorph ometric and other analyses were obtained in all samples obtained. The percutaneous muscle biopsy performed with enhanced suction using inexp ensive, readily available nipples enhances tissue yield two- to fivefo ld.