Application of electrophoresis technology to DNA analysis

We used the variable number tandem repeat (VNTR) polymorphism and the ten s hort tandem repeat (STR) polymorphisms to study a number of disputed patern ity cases in the Japanese population. For the determination of VNTR locus ( D1S80) and the ten STR loci (vWA, F13B, TH01, TPOX, CSF1PO, F13A01, LPL, D3 S1744, D12S1090, D18S849) we used polymerase chain reaction (PCR) amplifica tion and the vertical polyacrylamide gel electrophoresis technique followed by SYBR green I staining. The irregular repeats were analyzed by sequencin g from bands of vertical polyacrylamide gel electrophoresis using the lates t gene analyzing equipment, the ABI PRISM 310 Genetic Analyzer. The probabl e genotypes of the deceased putative father were deduced by Komatu's method from the genotypes of the widow and the genotypes of their children. The c alculation of paternity probability used the Essen-Moller formula and Bayes 's theorem. Calculated in eleven loci, the distinguishing probabilities (DP ) and the mean exclusion chance (MEC) were 0.9999 and 0.9989, respectively. Therefore, information obtained from eleven DNA polymorphisms is enough to determine paternity plausibility.