E. Litborn et al., Parallel reactions in open chip-based nanovials with continuous compensation for solvent evaporation, ELECTROPHOR, 21(1), 2000, pp. 91-99
In an earlier report (Litborn, E., Emmer,,Angstrom., Roeraade, J., Anal. Ch
im. Acta 1999, 401, 11-19, we described a technique for performing chemistr
y in chip-based vials. A major problem, solvent evaporation, was partially
remedied by using a closed humidity chamber. in this paper we report an imp
roved technique for performing parallel reactions in open, 15 nL volume, ch
ip-based vials. The evaporation of solvent from the reaction fluid was cont
inuously compensated by addition of solvent via an array of microcapillarie
s. The suitability of the method was demonstrated by performing eight separ
ate peptide maps of myoglobin in parallel, using the three enzymes trypsin,
alpha-chymotrypsin and endoproteinase Glu-C. The total amount of myoglobin
utilized to perform the eight digests was less than 100 pmol. The correspo
nding amount of enzymes was ca. 0.1 pmol per reaction. In order to evaluate
the operating limits of the technique, a study of the evaporation of solve
nts from a series of vials with proportionally smaller volumes operated at
different temperatures was performed. The results showed that the concept f
or continuous compensation of solvent evaporation should be applicable to r
eaction volumes down to 30 pL.