Capillary zone electrophoresis with electroosmotic flow controlled by external radial electric field

A new way of regulation of electroosmotic flow (EOF) in capillary zone elec trophoresis (CZE) by external electric field has been developed. A set of t hree high-voltage power supplies is used to form a radial electric field ac ross the capillary wall. One power supply is applied in the usual way as a driving force of CZE and EOF to the ends of the inner capillary compartment dipped into the electrode vessels and filled with background electrolyte. Two power supplies are connected to the ends of the outer low-conductivity coating of the capillary which is formed by the dispersion of copolymer of aniline and p-phenylenediamine in polystyrene matrix. The difference betwee n electric potentials on the outer capillary surface and inside the capilla ry determines the voltage of radial electric field across the capillary wal l and affects the electrokinetic potential at the solid-liquid interface in side the capillary. The effect of magnitude and polarity of external radial electric field on the flow rate of EOF, on the migration times of charged analytes and on the separation efficiency and resolution of CZE separations of synthetic oligopeptides, diglycine, triglycine and octapeptide fragment s of human insulin was evaluated. Through the EOF control by external elect ric field the dynamic effective length of the capillary was obtained and th e speed of analysis and resolution of CZE separations of peptide analytes c ould be optimized.