A colicin-tolerant Escherichia coli mutant that confers Hfl phenotype carries two mutations in the region coding for the C-terminal domain of FtsH (HflB)

An Escherichia coli mutant, ER437, which was originally isolated for colici n tolerance, was found to carry two amino acid changes in the C-terminal po rtion of FtsH (HflB). These mutations were demonstrated to reduce the abili ty of FtsH ro degrade the phage lambda CII protein in vivo and in vitro, pr oviding a rationalization for the mutant Hn phenotype. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. A ll rights reserved.