Control of penaeid acute viremia (PAV) in Penaeus japonicus: Selection of eggs based on the PCR detection of the causative virus (PRDV) from receptaculum seminis of spawned broodstock

In order to prevent penaeid acute viremia (PAV) in seed production of kurum a prawn Penaeus japonicus, the presence of penaeid rod-shaped DNA virus (PR DV) in the ovary and receptaculum seminis was examined by polymerase chain reaction (PCR). The detection rate of PRDV was always higher in the recepta culum seminis than in the ovary. The prevalence of the virus in the recepta culum seminis became higher after spawning. especially in the spawners that required a longer period to induce spawning. PAV occurred in postlarvae cu ltured at Kamiura Station of Japan Sea-Farming Association in 1997 when seg regation of spawners was made based on the results of PRDV detection from o varies before spawning, whereas the disease did not occur in 1998 or 1999 w hen segregation of eggs was performed according to the result of virus dete ction from the receptaculum seminis after spawning. These results indicate that the selection of eggs should be done in the hatchery based on the resu lts of PCR detection from receptaculum seminis of spawners after spawning.