Two species of Bromus adapted to different climatic conditions were st
udied to determine their responses to chilling and water stress. Enzym
es of sucrose and fructan metabolism as well as fructoside content wer
e measured in roots and shoots. The results indicate that Bromus pictu
s (adapted to a cold desert area) has a constitutive fructan synthesis
, whilst B. auleticus (adapted to a warmer northern climate) only accu
mulated fructan under cold stress. Microscopic observations of roots e
xposed to fluorescent probes ascertained cell viability and showed tha
t the apex of roots in the northern species were more affected by the
water-deficit treatment than the southern species. The presence of fru
ctans in B. pictus and their absence in B. auleticus support the hypot
hesis that fructans play a role in plant tolerance to environmental st
ress.