Detrimental effect of the combination of R164S with G238S in TEM-1 beta-lactamase on the extended-spectrum activity conferred by each single mutation

Authors
Giakkoupi, P Tzelepi, E Tassios, PT Legakis, NJ Tzouvelekis, LS
Citation
P. Giakkoupi et al., Detrimental effect of the combination of R164S with G238S in TEM-1 beta-lactamase on the extended-spectrum activity conferred by each single mutation, J ANTIMICRO, 45(1), 2000, pp. 101-104
Citations number
10
Categorie Soggetti
Pharmacology,Microbiology
Journal title
Journal of antimicrobial chemotherapy
ISSN journal
03057453 → ACNP
Volume
45
Issue
1
Year of publication
2000
Pages
101 - 104
Database
ISI
SICI code
Abstract
The non-naturally occurring TEM-1 beta-lactamase mutant R164S:G238S, as wel l as the R164S (TEM-12) and G238S (TEM-19) beta-lactamases, were constructe d and expressed in Escherichia coli under isogenic conditions. Comparison o f susceptibilities to beta-lactam antibiotics and substrate profiles showed that the combination of R164S with G238S drastically reduced the extended- spectrum activity of the respective single mutants.