M. Tessari et Gw. Vuister, A novel experiment for the quantitative measurement of CSA(H-1(N))/CSA(N-15) cross-correlated relaxation in N-15-labeled proteins, J BIOM NMR, 16(2), 2000, pp. 171-174
An experiment is presented which allows for the quantitative measurement of
the relaxation interference between the H-1(N) CSA and N-15 CSA interactio
ns in N-15 labeled proteins. A constant-time buildup scheme is used to meas
ure the differential relaxation rate, eta, between double-quantum (DQ) and
zero-quantum (ZQ) H-1(N)-N-15 coherences. The CSA/CSA experiment was record
ed at three different B-o field strengths. The CSA(H-1(N))/CSA(N-15) cross-
correlation rate was obtained from the linear fit of the measured rate, eta
, versus B-o(2) for 77 residues of the EH2 domain from mouse Eps15.