Gm. De Courten-myers et al., Hypoglycemic brain injury: Potentiation from respiratory depression and injury aggravation from hyperglycemic treatment overshoots, J CEREBR B, 20(1), 2000, pp. 82-92
Hypoglycemia can cause brain dysfunction, brain injury, and death. The pres
ent study seeks to broaden current information regarding mechanisms of hypo
glycemic brain injury by investigating a novel etiology. The cat's high res
istance to brain injury from hypoglycemia suggested that additional influen
ces such as respiratory depression might play a facilitating role. Three gr
oups of cats were exposed to fasting and insulin-induced hypoglycemia (HG;
n = 6), euglycemic respiratory depression (RD; n = 5), and combined hypogly
cemic respiratory depression (HG/RD; n = 10). The HG animals were maintaine
d at <1.5 mmol (mean I mmol) serum glucose concentration for 2 to 6.6 hours
. The respiratory depression was associated with Pao, and Pace, values of s
imilar to 50 mm Hg for hour and of similar to 35 and similar to 75 mm Hg, r
espectively, for the second hour. Magnetic resonance diffusion-weighted ima
ging estimated brain energy state before, during, and after hypoglycemia. T
he hypoglycemic respiratory depression exposures were terminated either to
euglycemia(n = 4) or to hyperglycemia (n = 6). Brain injury was assessed af
ter 5 to 7 days of survival. Cats exposed to hypoglycemia alone maintained
unchanged diffusion coefficients; that is, they lacked evidence of brain en
ergy failure and all six remained brain-intact. Only 1 of 5 euglycemic RD b
ut 10 of 10 HG/RD cars developed brain damage (HG and RD vs. HG/RD, P < 0.0
1). This difference in brain injury rates suggests injury potentiation by h
ypoglycemia and respiratory depression acting together. Three injury patter
ns emerged, including activation of microglia, selective neuronal necrosis,
and laminar cortical necrosis, Widespread activation of microglia suggesti
ng damage to neuronal cell processes affected all damaged brains. Selective
neuronal necrosis affecting the cerebral cortex, hippocampus, and basal ga
nglia was observed in all but one case, instances of laminar cortical necro
sis were limited to cats exposed to hypoglycemic respiratory depression tre
ated with hyperglycemia. Thus, treatment with hyperglycemia compared with e
uglycemia after hypoglycemic respiratory depression exposures significantly
increased the brain injury scores (24 +/- 6 vs. 13 +/- 2 points: P < 0.05)
. This new experimental hypoglycemia model's contribution lies in recognizi
ng additional factors that critically define the occurrence of hypoglycemic
brain injury.