It is shown that supersymmetry is spontaneously broken in certain three-dim
ensional supersymmetric gauge theories, by using the s-rule in their string
theory realization as brane configurations. In particular, supersymmetry i
s broken in N = 3 supersymmetric Yang-Mills-Chern-Simons theory with gauge
group SU(n) and CS coefficient k, as well as in its N = 2 and N = 1 deforma
tions, when n > \k\. In addition, supersymmetry is broken in the N = 1 mass
deformation of N = 2 supersymmetric Yang-Mills theory with gauge group SU(
n) and one matter multiplet when n >1. In the latter case the breaking is i
nduced by an instanton-generated repulsive potential.