Relationship between tree nitrogen status, xylem and phloem sap amino acidconcentrations, and apparent soil nitrogen uptake by almond trees (Prunus dulcis)

Four N application rates generated a range of tree N status as indicated by leaf N concentrations in six year old trees of 'Mission' almond (Prunus du lcis (Mill.) D. A. Webb), Leaf N concentrations were related positively wit h concentrations of amino acids - especially asparagine and glutamine - in phloem and xylem saps. A pulse of 1.25 kg N per tree was applied to trees p reviously receiving 0, 78, 157 or 313 g N per tree, annually. Apparent upta ke of soil N into above-ground tree parts was determined over a 39 d period . N uptake was determined as the difference in the products of tree biomass and tissue N concentrations before and after the N pulse. Tissue N concent rations increased significantly in trees previously receiving the two lowes t N application rates, whereas trees previously receiving the high N treatm ents were unresponsive. Tree N contents (0 g N per year, and also 78 g N pe r year treatments) increased slightly in response to the N pulse, although statistical differences were not observed due possibly to high variability in biomass among trees. At the same time, the amino acid concentrations in phloem exudate and xylem sap were highest in trees grown under the highest N fertilization regime. We suggest that the high amino acid concentrations in the phloem and xylem saps are indicative of a larger pool of amino N cyc ling throughout the vasculature of high N-status trees. Our data indicate a n inverse relationship between tree N status, amino acid concentrations in xylem and phloem saps and capacity for soil N uptake in field-grown trees. These results represent the first field data to correlate the concentration of amino acids circulating in the plant vasculature with soil N uptake. A functional relationship between the amino acid concentrations cycling withi n the tree and the regulation of soil N uptake has, however, not yet been e stablished.