New N-15 NMR exchange experiments for the unambiguous assignment of H-1(N)/N-15 resonances of proteins in complexes in slow chemical exchange with free form

Authors
Vialle-Printems, C van Heijenoort, C Guittet, E
Citation
C. Vialle-printems et al., New N-15 NMR exchange experiments for the unambiguous assignment of H-1(N)/N-15 resonances of proteins in complexes in slow chemical exchange with free form, J MAGN RES, 142(2), 2000, pp. 276-279
Citations number
19
Categorie Soggetti
Chemistry & Analysis","Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF MAGNETIC RESONANCE
ISSN journal
10907807 → ACNP
Volume
142
Issue
2
Year of publication
2000
Pages
276 - 279
Database
ISI
SICI code
1090-7807(200002)142:2<276:NNNEEF>2.0.ZU;2-B
Abstract
The potentialities of a 2D proton-detected heteronuclear exchange experimen t to assign the nitrogen and amide proton resonances in a uniformly N-15-en riched macromolecule involved in a complex, starting from the free form ass ignments, are demonstrated on a protein-DNA complex. This 2D experiment is further extended to a 3D experiment in the case of severe superpositions. ( C) 2000 Academic Press.