G. Muller-strahl et al., Inhibition of nitric oxide synthase augments the positive inotropic effectof nitric oxide donors in the rat heart, J PHYSL LON, 522(2), 2000, pp. 311-320
1. In this investigation we studied the effects of nitric oxide on contract
ility and heart rate in normal saline-perfused rat hearts where shear stres
s-induced endothelial NO synthesis substantially contributes to total cardi
ac NO production. In addition, we sought to estimate the concentrations of
exogenous NO producing inotropic effects.
2. We investigated the effects of glyceryl trinitrate (GTN), S-nitroso-D,L-
penicillamine (SNAP), sodium (Z)-1-(N;N-diethylamino)diazen-1-ium-1,2-diola
t (DEA/NO), and DEA/NO in the presence of the NO synthase inhibitor N-omega
-nitro-L-arginine (L-NA) in constant-flow-perfused spontaneously beating ra
t Langendorff hearts and in rat working hearts.
3. In Langendorff hearts, GTN (10 nM to 100 mu M, n = 32) induced a positiv
e inotropic response that plateaued at 1 mu M GTN with a maximal rate of in
crease of left ventricular pressure during ventricular contraction (+dP/dt(
max)) of 6.33 +/- 2.56% (n = 11, P < 0.5). Similarly, both spontaneous NO d
onors (0.1. nM to 1 mu M, corresponding to approximately 0.03-0.3 mu M NO)
induced a positive inotropic response of 10.6 +/- 3.1 % (SNAP; n = 15, P <
0.05) and 11.5 +/- 2.7 % (DEA/NO, n = 15, P < 0.05).
4. The positive inotropic effect of SNAP and DEA/NO progressively declined
from 1 mu M to 100 mu M Of the NO donors (corresponding to approximately 0.
3-30 mu M NO).
5. In the isolated working rat heart, 0.1 mu M DEA/NO induced an increase o
f +dP/dt(max) of 7.5 +/- 2.5% (n = 9, P < 0.05). Inhibition of NO synthase
by L-NA produced a 4-fold increase in this effect of DEA/NO.
6. We suggest that physiological NO concentrations support myocardial perfo
rmance. In normal rat hearts the positive inotropic effect of NO appears to
be almost maximally exploited by the endogenous NO production.