BACULOVIRUS TRANSACTIVATOR IEL IS FUNCTIONAL IN MAMMALIAN-CELLS

IE1 of Autographa californica multicapsid nuclear polyhedrosis virus a cts as a transactivator of several viral promoters in insect cells. Tr ansient expression assays indicate that IE1 is involved in the activat ion of the early promoter he65 in both insect TN-368 and mammalian BHK -21 cell lines. IE1 activation of the he65 promoter was compared with IE1 activation of the early 39K promoter. In contrast to the he65 prom oter, the 39K promoter was not inducible by IE1 in BHK-21 cells.