P. Villalonga et al., [Lys(61)]N-Ras is able to induce full activation and nuclear accumulation of Cdk4 in NIH3T3 cells, ONCOGENE, 19(5), 2000, pp. 690-699
The elements of the cell cycle regulatory machinery activated by the oncoge
nic form of Ras, [Lys(61)]N-Ras, have been analysed in NIH3T3 cells. We dem
onstrate that [Lys(61)]N-Ras expression is able to induce full cdk4 activat
ion. As already reported, oncogenic Ras expression was sufficient to induce
cyclin D1 and p21(cip1) expression and their association with cdk4. Furthe
rmore, serum-starved [Lys(61)]N-Ras NIH3T3 cells showed nuclear accumulatio
n of cyclin D1 and cdk4 not observed in serum-started NIH3T3 cells. This ac
cumulation of cdk4 into the cell nucleus observed in serum-started [Lys(61)
]N-Ras NIH3T3 cells was inhibited by a microinjection of neutralizing anti-
Ras antibodies. Thus, active [Lys(61)]N-Ras was a sufficient signal to indu
ce nuclear accumulation of cyclin D1/cdk4, leading to its full activation,
Transfection of [Lys(61)]N-Ras NIH3T3 cells with an inactive form of MEK or
their treatment with PD 98059, showed that nuclear translocation of cdk4 w
as MEK dependent. Interestingly, cells constitutively expressing [Lys(61)]N
-Ras did not inactivate pRb and did not proliferate in the absence of serum
. This may be due to the fact that although association of cdk2 with cyclin
E and the translocation of those complexes to the nucleus were achieved, [
Lys(61)]N-Ras expression was not sufficient to induce cdk2 activation. The
high levels of p27(kip1) that were found in cyclin E/cdk2 complexes may be
responsible for the inability of oncogenic Res to activate this kinase. In
consequence, oncogenic alterations that lead to a decrease in p27(kip1) bou
nd to cyclin E may cooperate with Ras to induce full cdk2 activation, pRb i
nactivation and thus cell proliferation.