Overproduction in Escherichia coli and purification of the hemolytic protein sticholysin II from the sea anemone Stichodactyla helianthus

Authors
de los Rios, V Onaderra, M Martinez-Ruiz, A Lacadena, J Mancheno, JM del Pozo, AM Gavilanes, JG
Citation
V. De Los Rios et al., Overproduction in Escherichia coli and purification of the hemolytic protein sticholysin II from the sea anemone Stichodactyla helianthus, PROT EX PUR, 18(1), 2000, pp. 71-76
Citations number
23
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PROTEIN EXPRESSION AND PURIFICATION
ISSN journal
10465928 → ACNP
Volume
18
Issue
1
Year of publication
2000
Pages
71 - 76
Database
ISI
SICI code
1046-5928(200002)18:1<71:OIECAP>2.0.ZU;2-9
Abstract
The cDNA coding for the cytolytic toxins sticholysin I and sticholysin II f rom the sea anemone Stichodactyla helianthus has been isolated, cloned in p UC18, and sequenced, A 6His-tagged version of sticholysin II has been overp roduced in Escherichia coli and purified to homogeneity in milligram amount s. Conformational and functional analyses of recombinant sticholysin II do not reveal any significant difference when compared to the natural cytolysi n, (C) 2000 Academic Press.