Heat-shock proteins associated with base excision repair enzymes in HeLa cells

Two enzymes of base excision repair (BER), uracil DNA glycosylase (UDG) and DNA polymerase beta (beta pol), from HeLa cells co-eluted from Superose 12 FPLC columns. The UDG was completely displaced from 150-180-kDa fractions to 30-70-kDa fractions by brief treatment with 0.5 N NaCl, pH 3.0, as expec ted when protein-protein associations are disrupted, but beta pol was not d isplaced by this treatment. UDG was not essential to the presence of beta p ol in the 150-180-kDa enzyme complex. beta pol and UDG apparently reside in separate but co-eluting structures. Immunoaffinity chromatography showed t hat the association of UDG and beta pol was accounted for by attachment in common to DNA and that the association was abolished by eliminating DNA, Ev idence for base excision repairosomes containing UDG and beta pol in protei n-protein assemblies was not found. However, UDG and human AP endonuclease (HAP1) were associated with HSP70 and HSP27, which are present in 150-180-k Da and 30-70-kDa proteins of cell sonicates, The association of HSPs with B ER enzymes was confirmed by hydroxyl radical protein-protein footprinting a nd immunoaffinity tests. The association of HSPs and BER enzymes is a novel finding. HSP binding may account for the presence of BER enzymes in the tw o large size class fractions and HSPs may have functional roles in BER. (C) 2000 by Radiation Research Society.