Vm. Morris et al., OPTIMIZATION OF THE CAPILLARY ELECTROPHORESIS SEPARATION OF RANITIDINE AND RELATED-COMPOUNDS, Journal of chromatography, 766(1-2), 1997, pp. 245-254
Citations number
20
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Experimental designs were used as part of a strategy to determine the
significant factors controlling separation and the optimum conditions
for analysis of ranitidine and related compounds by using capillary el
ectrophoresis. Two-level fractional factorial designs were employed to
screen for the significant factors in the separation with six factors
studied. It was necessary to use two fractional factorial designs due
to significant overall curvature in the first of the designs used in
the screening. Central composite designs were applied to determine the
optimal conditions for the significant factors. Multi-linear regressi
on and canonical analysis were used in the analysis. The optimization
was based on a response function which takes into account all the peak
s in the electropherogram. The pH value and the voltage were found to
be the most significant factors.