Cardiolipin antibodies dependant on beta2-glycoprotein-I in antiphospholipid syndrome

Aim. To study beta2-GP-I-dependant binding of phospholipid antibodies (PAb) to phospholipids and this process participation in pathogenesis of antipho spholipid syndrome (APS). Materials and methods. IgG-fractions and sera from 20 patients with APS. Co factor activity of beta2-GP-I isolated from serum of healthy donors was exa mined with modified immunoassay. Results. Contrary to donor IgG, binding of IgG fractions isolated from sera of APS patients with cardiolipin grows dose-dependently in the presence of beta2-GP-I. Cofactor activity of beta2-GP-I is confirmed in the study of s era of APS patients. Sera containing beta2-GP-I-dependant antibodies to car diolipin (aCL), unlike a CL-negative sera, react with solid-phase immobiliz ed beta2-GP-I. Conclusion. It is confirmed that beta2-GP-I participates in interaction of PAb with cardiolipin. Pathogenetic implication of beta2-GP-I-dependant PAb for onset of APS is discussed.