This paper describes the natural occurrence of the toxin, cylindrospermopsi
n, in two species of cyanobacteria found in Australia. The structure and ch
emical properties of this compound are described along with a nontoxic anal
og of cylindrospermopsin. The results of both intraperitoneal (IP) and oral
dosing of mice show that hepatotoxicity is the main effect of cylindrosper
mopsin in vivo, but that a thrombo-hemorrhagic phenomenon is observed in a
proportion of dosed animals. It has been shown that the toxin can be metabo
lized in vivo and that a bound metabolite occurs in the liver. Cytotoxicity
experiments using cell cultures show that cylindrospermopsin is more cytot
oxic to isolated rat liver hepatocytes than to other cell types. Risk asses
sment calculations show that guideline values for cylindrospermopsin in dri
nking water should lie in the low microgram per liter range.