Influence of batroxobin on cerebral ischemia-reperfusion injury in gerbils

AIM: To study the effects of batroxobin (Bat) on neurons survival, neurobeh avioral test, ATP levels and hydroxyl radical outputs in hippocampus during forebrain ischemia-reperfusion in gerbils. METHODS: The forebrain ischemia was induced by occluding the bilateral common carotid arteries for 10 min in gerbils, and ATP levels and 2, 3-dihydroxybenzoic acid (DHBA) outputs we re assayed by HPLC. The neurons survival were assessed by histology, and be havioral tests of gerbils were assessed by open field test. RESULTS: The nu mber of neurons survival-in Lr at d 7 postischemic insult were (7 +/- 4) % of sham-operated gerbils, much less than that in Bac( 45 +/- 16) %. The lev els of explore activities of ischemic gerbils was 175 % and 159 % of sham-o perated gerbils:at d 3 and d 6 postischemic insult, much more than that in Bat (120 % d 3 and 140 % d 6). Hippocampal ATP levels in Ir were 64 % of sh am-operated gerbils at reperfusion 60 min, much less than that in Bat I and II (82 % and 89 % respectively). The hippocampal 2,3-DHBA outputs in Ir in creased by 4.5 folds of sham-operated gerbils at reperfusion 60 min, but th e 2,3-DHBA outputs in Bat I and Bat II were only 2.6 and 2.4 folds respecti vely. CONCLUSION: Bat possesses the inhibitory effects on DND and OH. produ ction following cerebral ischemia-reperfusion in gerbils.